Title
Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana
Document Type
Article
Publication Date
5-2-2022
Abstract
Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.
Recommended Citation
Marzol, Eliana; Borassi, Cecilia; Sardoy, Mariana Carignani; Ranocha, Philippe; Aptekmann, Ariel A.; Bringas, Mauro; Pennington, Janice; Paez-Valencia, Julio; Pacheco, Javier Martínez; Rodríguez-Garcia, Diana R.; Guerrero, Yossmayer Del Carmen Rondón; Peralta, Juan Manuel; Fleming, Margaret; Mishler-Elmore, John W.; Mangano, Silvina; Blanco-Herrera, Francisca; Bedinger, Patricia A.; Dunand, Christophe; Capece, Luciana; Nadra, Alejandro D.; Held, Michael; Otegui, Marisa S.; and Estevez, José M., "Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana" (2022). Chemistry & Biochemistry Open Access Publications. 42.
https://ohioopen.library.ohio.edu/chemistry-oapub/42