Surveying helix 12 dynamics within constitutively active estrogen receptors using bipartite tetracysteine display

Presenter Information

Lasantha Sendanayake

Abstract

Our laboratory recently developed fluorescence-based assay that involves a genetically encodable tetracysteine (C4) tag coupled with a biarsenical profluorophore FlAsH-EDT2 to assess H12 dynamics of Estrogen Receptor (ER) in real-time and it was used in this study to asses dynamic nature of constitutively active ER in the presence of two breast cancer mutations.

Status

Graduate

Department

Chemistry & Biochemistry

College

College of Arts and Sciences

Campus

Athens

Faculty Mentor

Holub, Justin

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Surveying helix 12 dynamics within constitutively active estrogen receptors using bipartite tetracysteine display

Our laboratory recently developed fluorescence-based assay that involves a genetically encodable tetracysteine (C4) tag coupled with a biarsenical profluorophore FlAsH-EDT2 to assess H12 dynamics of Estrogen Receptor (ER) in real-time and it was used in this study to asses dynamic nature of constitutively active ER in the presence of two breast cancer mutations.