Surveying helix 12 dynamics within constitutively active estrogen receptors using bipartite tetracysteine display
Abstract
Our laboratory recently developed fluorescence-based assay that involves a genetically encodable tetracysteine (C4) tag coupled with a biarsenical profluorophore FlAsH-EDT2 to assess H12 dynamics of Estrogen Receptor (ER) in real-time and it was used in this study to asses dynamic nature of constitutively active ER in the presence of two breast cancer mutations.
Status
Graduate
Department
Chemistry & Biochemistry
College
College of Arts and Sciences
Campus
Athens
Faculty Mentor
Holub, Justin
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Surveying helix 12 dynamics within constitutively active estrogen receptors using bipartite tetracysteine display
Our laboratory recently developed fluorescence-based assay that involves a genetically encodable tetracysteine (C4) tag coupled with a biarsenical profluorophore FlAsH-EDT2 to assess H12 dynamics of Estrogen Receptor (ER) in real-time and it was used in this study to asses dynamic nature of constitutively active ER in the presence of two breast cancer mutations.